A collection of fossil material from Eocene deposits of Pakistan includes remains belonging to various taxa of actinopterygian fishes. The material comes from predominantly freshwater deposits, as well as a few marine deposits, in the Kuldana Formation in the Kala Chitta Hills, and from the Chorgali Formation near Gali Jaghir, in Attock District of Punjab Province. The Kuldana and Chorgali formations are early to middle Eocene in age. Isolated bones and teeth are identified as belonging to Eotrigonodontidae, Pycnodontiformes, Amiidae, Osteoglossidae, Siluriformes (cf. Bagridae), and Perciformes, as well as unidentified Teleostei. Some of the fossils are skulls of a channiform fish, which is here described as a new genus and species, Anchichanna kuldanensis. The faunal affinities of the Indo-Pakistan area during the Eocene are not clear, with previous studies suggesting it is faunally more similar to other parts of modern Asia, others as it representing a region of endemism, or even as being similar to northern Africa. The fish material reported here indicates a relationship between the Pakistan fauna in the Eocene with that of northern Africa.